Human Irisin/FNDC5 ELISA Kit PicoKine®

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 893 3980 278 1073 3141 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 893 3980 278 1073 3141 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 893 3980 278 1073 3141 Mean (pg/ml) 162 893 3980 278 1073 3141 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 Standard deviation 9.19 65.01 265.47 13.79 55.58 243.11 CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7% CV (%) 5.7% 7.3% 6.7% 5.0% 5.2% 7.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969 Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969 Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969 O.D. 0.035 0.065 0.114 0.219 0.386 0.703 1.252 1.969

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.