Anti-Pro/Actived Complement C1s Antibody

Our mouse Pro/Actived Complement C1s monoclonal antibody from Antibodies Incorporated is produced in-house from clone 49. It detects human Pro/Actived Complement C1s and is Protein A or Protein G purified. Works in ELISA, IF.

Product Specifications

Background

C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes but also by other cells. The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa) . The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.

Synonyms

C1S, EDSPD2, complement C1s subcomponent, C1 esterase, basic proline-rich peptide IB-1, complement component 1 subcomponent s, complement component 1, s subcomponent

Gene Name

C1S

NCBI Gene ID

716

UniProt

P09871

Host

Mouse

Reactivity

Human

Immunogen

Human plasma C1s Proenzyme

Target

Pro/Actived Complement C1s

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

Type

Primary Antibodies

Applications

ELISA, IF

Concentration

1 mg/mL ±15%

Format

Protein A or Protein G purified

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Molecular Weight

Pro-enzyme C1s (pro-C1s) appears as a single-chain polypeptide around 80 kDa. Upon activation, it is cleaved into two disulfide-linked chains: a ~57 kDa A-chain and and 27 kDa B-chain) .