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Anti-Arl13b (N295B/66) Recombinant Guinea Pig Chimeric mAb FL650 Conjugate

Selected Variant

74-287-FL650

Size: 200 µL

Our guinea pig Anti-Arl13b FL650 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N295B/66. It is KO validated, detects human, mouse, non-human primate, rat, and zebrafish Arl13b, and is purified by affinity chromatography. It works in ICC, IHC.

Product Specifications

Background

ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1. Arl13B is a small GTPase with both N-terminal and C-terminal guanine nucleotide-binding motifs. Arl13B is localized to the membrane of cilia and expressed in Neuroepithelial cells and developing radial glia of the developing cerebral cortex. Mutations in this gene are the cause of Joubert syndrome 8.

Synonyms

ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) (Protein hennin)

Gene Name

Arl13b Arl2l1

UniProt

Q640N2

Host

Guinea Pig

Reactivity

Human, Mouse, Non-Human Primate, Rat, and Zebrafish

Immunogen

Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (accession number Q640N2) produced recombinantly in E. Coli

Target

Arl13b

Clonality

Recombinant

Isotype

IgG

Clone

N295B/66

Type

Primary Antibodies

Applications

ICC, IHC

Concentration

0.5 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

50 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N295B/66 with guinea pig IgG heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N295B/66 but can be detected using standard anti-guinea pig secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Specificity

Does not cross-react with Arl13a (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Brand

Antibodies Inc

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Mouse

Conjugate

FL650 Ex: 655 nm, Em: 676 nm

Price

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