Human CD59 ELISA Kit PicoKine®

CD59 glycoprotein, also known as MAC-inhibitory protein (MAC-IP), membrane inhibitor of reactive lysis (MIRL), or protectin, is a protein that in humans is encoded by the CD59 gene. This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

16.3A5; 1F5; 1F5 antigen; CD59; CD59 glycoprotein; EJ16; EJ30; EL32; G344; HRF 20; HRF20; MAC inhibitory protein; MAC IP; MACIF; MEM43; MEM43 antigen; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18 20; Protectin

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 375 1985 88 387 1400 Standard deviation 1.68 22.28 118.90 3.84 15.48 108.08 CV (%) 2.6% 5.9% 6.0% 4.4% 4.0% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 375 1985 88 387 1400 Standard deviation 1.68 22.28 118.90 3.84 15.48 108.08 CV (%) 2.6% 5.9% 6.0% 4.4% 4.0% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 64 375 1985 88 387 1400 Mean (pg/ml) 64 375 1985 88 387 1400 Standard deviation 1.68 22.28 118.90 3.84 15.48 108.08 Standard deviation 1.68 22.28 118.90 3.84 15.48 108.08 CV (%) 2.6% 5.9% 6.0% 4.4% 4.0% 7.7% CV (%) 2.6% 5.9% 6.0% 4.4% 4.0% 7.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.010 0.079 0.135 0.235 0.416 0.758 1.277 1.924 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.010 0.079 0.135 0.235 0.416 0.758 1.277 1.924 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.010 0.079 0.135 0.235 0.416 0.758 1.277 1.924 O.D. 0.010 0.079 0.135 0.235 0.416 0.758 1.277 1.924

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.