Human G-CSF High Sensitivity ELISA Kit PicoKine®

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 8.86 34.39 120.48 7.83 24.3 100.58 Standard deviation 0.240 1.024 9.554 0.211 0.880 4.526 CV (%) 2.7% 3.0% 7.9% 2.7% 3.6% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 8.86 34.39 120.48 7.83 24.3 100.58 Standard deviation 0.240 1.024 9.554 0.211 0.880 4.526 CV (%) 2.7% 3.0% 7.9% 2.7% 3.6% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 8.86 34.39 120.48 7.83 24.3 100.58 Mean (pg/ml) 8.86 34.39 120.48 7.83 24.3 100.58 Standard deviation 0.240 1.024 9.554 0.211 0.880 4.526 Standard deviation 0.240 1.024 9.554 0.211 0.880 4.526 CV (%) 2.7% 3.0% 7.9% 2.7% 3.6% 4.5% CV (%) 2.7% 3.0% 7.9% 2.7% 3.6% 4.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558