Rat TNF Alpha/Tumor necrosis factor High Sensitivity ELISA Kit PicoKine®

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2.97 11.67 40.62 2.79 11.85 32.84 Standard deviation 0.059 0.447 2.019 0.136 0.558 1.593 CV (%) 2.0% 3.8% 5.0% 4.9% 4.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2.97 11.67 40.62 2.79 11.85 32.84 Standard deviation 0.059 0.447 2.019 0.136 0.558 1.593 CV (%) 2.0% 3.8% 5.0% 4.9% 4.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2.97 11.67 40.62 2.79 11.85 32.84 Mean (pg/ml) 2.97 11.67 40.62 2.79 11.85 32.84 Standard deviation 0.059 0.447 2.019 0.136 0.558 1.593 Standard deviation 0.059 0.447 2.019 0.136 0.558 1.593 CV (%) 2.0% 3.8% 5.0% 4.9% 4.7% 4.9% CV (%) 2.0% 3.8% 5.0% 4.9% 4.7% 4.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883 O.D. 0.041 0.089 0.125 0.18 0.274 0.475 0.958 1.883