What is the catalog number for (HRP) Broad Spectrum (DAB) Kit, 1 Kit (8 ml/80 tests)?

The catalog number for (HRP) Broad Spectrum (DAB) Kit, 1 Kit (8 ml/80 tests) is orb1087636.

What sizes are available for (HRP) Broad Spectrum (DAB) Kit, 1 Kit (8 ml/80 tests)?

(HRP) Broad Spectrum (DAB) Kit, 1 Kit (8 ml/80 tests) is available in the following size(s): 1 Unit.

Is (HRP) Broad Spectrum (DAB) Kit, 1 Kit (8 ml/80 tests) in stock?

(HRP) Broad Spectrum (DAB) Kit, 1 Kit (8 ml/80 tests) is currently made to order / not held in immediate stock. Contact us for lead time.

(HRP) Broad Spectrum (DAB) Kit, 1 Kit (8 ml/80 tests)

8 ml Peroxide Block (ready-to-use) 8 ml Blocking Solution Reagent 1 (ready-to-use) 8 ml Biotinylated Secondary Antibody, polyvalent Reagent 2 (ready-to-use) 8 ml Streptavidin-HRP-Conjugate Reagent 3 (ready-to-use) 7 x 5 ml DAB Substrate Buffer 3 ml DAB Concentrate (Chromogen) Substrate systems recommended (if not included in the kit) : Permanent AEC kit, AEC single solution, AEC substrate kit, DAB substrate kit, DAB high contrast kit. Materials required but not supplied Positive und negative control tissue Xylene or suitable substitutes Ethanol, distilled H2O 3% H2O2 solution Reagents for enzyme digestion or heat pre-treatment Wash buffer Pink PAP Pen Primary antibody (user-defined) Primary antibody diluent Negative control reagent Chromogenic substrate Counter stain solution Mounting medium Cover slips

Product Specifications

Storage Conditions

The solutions should be stored at 2-8°C without fur ther dilution. Please store the reagents in a dark place and do not freeze them. Under these conditions the solutions are stable up to the expiry date indicated on the label. They should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by the kit reagents, please contact our technical support.

Notes

For research use only.

Applications Notes

Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Endogenous peroxidase activity in the tissue may cause non-specific staining. This enzyme activity can be blocked by incubation with 3% H2O2-solution ("Peroxide Block") . Background staining caused by unspecific binding of the primary or secondary antibody is minimized by incubation with a protein blocking solution ("Blocking Solution") . This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the biotinylated secondary antibody is applied and incubated. This secondary antibody functions as a link between primary antibody and the streptavidin-horse radish peroxidase-conjugate ("Streptavidin-HRP-Conjugate") . A second washing is followed by the application of this conjugate. It binds to the biotin at the secondary antibody. Any excess of unbound streptavidin-HRP-conjugate is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the horse radish peroxidase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen AEC (included only in kit MON-APP114) leads to the formation of a red-brown product of reaction at the place of the target antigen. The chromogen DAB (included only in kit MON-APP115) forms a dark brown precipitate.