10X Pbs Ph 7.2 With Azide
Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution) . PBS can act as a diluent for many experimental preparations and is non-toxic to cells. This preparation contains 0.1% sodium azide as a preservative and is 10X concentrated to be diluted 10-fold to the working concentration.
Product Specifications
Product Name Alternative
Phosphate Buffered Saline
Conjugation
Unconjugated
Applications
ELISA: 1:10, IHC: 1:10, WB: 1:10
Concentration
10 X
Purity
10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. 10X PBS pH 7.2 with Azide was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Form
Liquid (sterile filtered)
Storage Conditions
Store container at room temperature (18° to 26° C) prior to opening. If desired, 10X PBS pH 7.2 with Azide may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.
Notes
For research use only.
Applications Notes
Phosphate Buffered Saline 10X pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X PBS pH 7.2 with Azide is meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Tested Applications
ELISA, IHC, WB
Preservative
Preservative: 0.1% (w/v) Sodium Azide; Buffer: See application note.
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