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10X Ttbs Ph 7.5

10X Ttbs Ph 7.5

Product Specifications

Product Name Alternative

TTBS, TBST, Tween Tris Buffered Saline

Conjugation

Unconjugated

Applications

ELISA: User Optimized, WB: User Optimized

Concentration

10X

Purity

10X TTBS solution was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Form

Liquid (sterile filtered)

Storage Conditions

Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

Notes

For research use only.

Applications Notes

Suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. Visit our newly expanded web site at www.biorbyt.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (v/v) Tween-20 at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.

Tested Applications

ELISA, WB

Preservative

Preservative: None. Stabilizer: None; Buffer: See application note.

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