20X Sspe Buffer

This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water treated with diethyl pyrocarbonate (DEPC) .