2X Sds-Page Sample Buffer Without Dtt Or B-Meoh

This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Store container at -20° C or colder prior to opening. Aliquot and re-freeze for best results. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue. The pH of this solution is 6.8. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.