ABTS 1-Component HRP Microwell Substrate (SUBA)
ABTS 1-Component HRP Microwell Substrate is a ready-to-use solution containing 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in ELISAs where the detection level is in the μg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP Microwell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors.
Product Specifications
Concentration
1 X
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Run ELISA according to the specific protocol through the conjugate incubation step. 2. Wash the wells three or four times with 1X ELISA Wash Buffer to remove any residual, AP-conjugate. 3. Bring SUBP to room temperature; protect from light. 4. Pipette 100 μL SUBP into each well of the plate. 5. Incubate SUBP 10-60 minutes. Monitor the color intensity. 6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 μL/well STOPP and read the samples between 405-420 nm within 1 hour.
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