AcalephFluor750 Conjugation Kit

1. Reaction Buffer, DMSO, Quencher reagent All reagents must be warmed to the room temperature before use. 2. AcalephFluor750 For label 1 mg protein size: Add 10 μl DMSO into the AcalephFluor750 tube. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette. The operation should be at room temperature, DMSO will turn to a solid at lower temperature. The dissolved reagent can be stored at -20°C for 2 weeks. For label 5 mg protein size: Add 50 μl DMSO into the AcalephFluor750 tube. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette. The operation should be at room temperature, DMSO will turn to a solid at lower temperature. The dissolved reagent can be stored at -20°C for 2 weeks. 3. Antibody The concentration of the antibody which need to be labeled, should be higher than 1 mg/ml. The lower concentration will affect conjugation efficiency.