AEC Peroxidase Chromogen Substrate Kit

(THIS KIT CONTAINS approximately 166 X CHROMOGEN, When diluted will make approx. 425 ml) ) 1. To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well. 2. Add one drop of reagent C chromogen, mix well. 3. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country’s regulations. Application: IHC, ICC 1. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/ deionized water. 2. Add few drops of ready to use AEC reagent, incubate at RT or 37oC for 5-10 minutes. 3. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water. 4. Add counterstain compatible with AEC, we use hematoxylin 30-60 seconds. 5. Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water. 6. Mount slide with aqueous mounting medium, we use our IHM please follow the protocol for mounting medium.