Antibody Diluent

Immunohistochemical staining procedures often start with incubation of a blocking solution to reduce unspecific binding of primary antibody to tissue sections. This step can be omitted if the antibody used is diluted in Antibody Diluent. Antibody Diluent  minimises unspecific binding of the primary antibody to the tissue section,  reduces surface tension of the antibody solution and improves spreading the reagent on the slide,  increases microbial and chemical stability of the antibody,  reduces adhesion of antibody to the surface of the vial,  and minimises the danger of antibody degradation by proteolytic enzymes.