Antibody Diluent B

Immunohistochemical staining procedures often start with incubation of a blocking solution to reduce unspecific binding of primary antibody to tissue sections. This step can be omitted if the antibody used is diluted in Antibody Diluent B. Antibody Diluent B • minimises unspecific binding of the primary antibody to the tissue section, • reduces surface tension of the antibody solution and improves spreading the reagent on the slide, • increases microbial and chemical stability of the antibody, • reduces adhesion of antibody to the surface of the vial, • and minimises the danger of antibody degradation by proteolytic enzymes.