Avidin

Avidin was prepared from chromatographically purified avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin. No reaction was observed against anti-Streptavidin.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Avidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Avidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized avidin.