Avidin Alkaline Phosphatase Conjugated
Avidin Alkaline Phosphatase Conjugated
Product Specifications
Product Name Alternative
Avidin Alkaline Phosphatase Conjugated, Avidin alk phos Conjugated
Conjugation
AP
Applications
ELISA: 1:8,000 - 1:32,000, FC: 1:500 - 1:2,500, IHC: 1:200 - 1:1,000, IF: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Purity
Avidin Alkaline Phosphatase Conjugated was prepared from electrophoretically pure Avidin from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-avidin.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Avidin Alkaline Phosphatase Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.
Tested Applications
ELISA, FC, IF, IHC, WB
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
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