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Avidin Alkaline Phosphatase Conjugated

Avidin Alkaline Phosphatase Conjugated

Product Specifications

Product Name Alternative

Avidin Alkaline Phosphatase Conjugated, Avidin alk phos Conjugated

Conjugation

AP

Applications

ELISA: 1:8,000 - 1:32,000, FC: 1:500 - 1:2,500, IHC: 1:200 - 1:1,000, IF: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Purity

Avidin Alkaline Phosphatase Conjugated was prepared from electrophoretically pure Avidin from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-avidin.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Avidin Alkaline Phosphatase Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

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