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Avidin Fluorescein Conjugated

Avidin Fluorescein Conjugated

Product Specifications

Product Name Alternative

Avidin Fluorescein Conjugated, Avidin FITC Conjugated

Conjugation

FITC

Applications

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Field of Research

Epigenetics

Concentration

2.2 mg/mL

Purity

Avidin Fluorescein Conjugated was prepared from chromatographical pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Avidin. No reaction was observed against anti-Streptavidin.

Bioactivity

3.55

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Avidin Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.

Tested Applications

DOT, FC, FLISA, IF

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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