What is the catalog number for Avidin/Biotin Blocking Kit Set?

The catalog number for Avidin/Biotin Blocking Kit Set is orb2652884.

What sizes are available for Avidin/Biotin Blocking Kit Set?

Avidin/Biotin Blocking Kit Set is available in the following size(s): 50 mL.

Is Avidin/Biotin Blocking Kit Set in stock?

Avidin/Biotin Blocking Kit Set is currently made to order / not held in immediate stock. Contact us for lead time.

Avidin/Biotin Blocking Kit Set

Some tissue sections contain endogenous biotin, biotin binding proteins, lectins or non-specific binding substances. Tissues may also bind avidin, biotin, peroxidase biotin, peroxidase streptavidin in avidin biotin based Immunohistochemistry. These tissues will give high background in the absence of biotinylated secondary antibodies. It may be necessary to block the tissue with avidin, followed by biotin. These reagents are added before the primary antibody.

Product Specifications

Field of Research

Cell Biology

Notes

For research use only.

Applications Notes

IHC/ICC procedure for frozen sections, paraffin sections, cell smears. 1. Deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols. (For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol) 2. Wash 2-3 with distilled or deionized water. 3. Wash slide with Tris/saline buffer, followed by blocking with normal blocking serum, rinse with buffer. 4. Block with reagent A (Avidin) for 5-10 minute, rinse with buffer. 5. Now block with reagent B (Biotin) for 5-10 minutes, wash thoroughly with buffer. Note: If antigen retriever is required it can be applied after this stage. 6. Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC) 3-5X. 7. Follow instructions for IHC/ICC.