BR Violet 540

Ghost Dye Violet 540 is provided in solution prepared in anhydrous DMSO and should be protected from light and moisture. Store vial at -20°C. Prior to use, allow vial to equilibrate to room temperature before opening. Ghost Violet 540 dye is stable through 20 freeze/thaw cycles, if needed, aliquot smaller volumes and store at -20°C. Cells labeled with Ghost Dye viability dyes can be cryopreserved for later use or used in intracellular staining protocols without any loss of fluorescence intensity. Ghost Dye Violet 540 has been quality-tested for flow cytometry using mouse bone marrow cells and can be used at 1 μL/mL of cell suspension. The concentration required for optimal performance should be determined empirically by investigator. Ghost Dye Violet 540 is excited by the violet (405 nm) laser line and has a peak emission of 537 nm that can be detected using a 525/50 band pass filter commonly used for detection of AmCyan.