Mouse GM-CSF High Sensitivity ELISA Kit PicoKine®

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2.78 8.67 45.71 2.25 7.03 47.24 Standard deviation 0.0734 0.458 3.542 0.07 0.29 2.182 CV (%) 2.6% 5.3% 7.8% 3.1% 4.1% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2.78 8.67 45.71 2.25 7.03 47.24 Standard deviation 0.0734 0.458 3.542 0.07 0.29 2.182 CV (%) 2.6% 5.3% 7.8% 3.1% 4.1% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2.78 8.67 45.71 2.25 7.03 47.24 Mean (pg/ml) 2.78 8.67 45.71 2.25 7.03 47.24 Standard deviation 0.0734 0.458 3.542 0.07 0.29 2.182 Standard deviation 0.0734 0.458 3.542 0.07 0.29 2.182 CV (%) 2.6% 5.3% 7.8% 3.1% 4.1% 4.6% CV (%) 2.6% 5.3% 7.8% 3.1% 4.1% 4.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.016 0.118 0.232 0.425 0.797 1.329 1.883 2.222 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.016 0.118 0.232 0.425 0.797 1.329 1.883 2.222 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (pg/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.016 0.118 0.232 0.425 0.797 1.329 1.883 2.222 O.D. 0.016 0.118 0.232 0.425 0.797 1.329 1.883 2.222