Cell Culture Contamination Detection Kit

Cell Culture Contamination Detection Kit. The cell culture contamination detection kit is not only used to detect the presence of contaminants but can also identify the type of contaminant, including yeast (and other fungi), Gram-positive bacteria, and Gram-negative bacteria. When using this kit, the sample needs to be stained on two slides: one slide is stained with Calcofluor White M2R, a blue fluorescent dye that specifically binds to fungal cell walls and can be excited by ultraviolet light. The other slide is stained with MycoLight Green JJ98 and Wheat Germ Agglutinin (WGA) -iFluor 594. JJ98 is a green fluorescent nucleic acid dye; WGA is a wheat germ agglutinin that can bind N-acetylglucosamine and N-acetylneuraminic acid residues. Since N-acetylglucosamine is generally more abundant and accessible in Gram-positive bacteria than in Gram-negative bacteria, the fluorescent conjugate of WGA can serve as a probe for Gram-positive bacteria. Therefore, the second slide can differentiate Gram-positive and Gram-negative bacteria. Gram-positive bacteria can be stained with both red and green fluorescence, whereas Gram-negative bacteria only exhibit green fluorescence.