Competent Cell Buffer
Competent Cell Buffer
Product Specifications
Product Name Alternative
Competent Cell Buffer, 60 mM Calcium Chloride, 15% (v/v) Glycerol, 10 mM Tris HCl, pH 7.5 Solution
Conjugation
Unconjugated
Concentration
1x
Purity
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Form
Liquid (sterile filtered)
Storage Conditions
Store container at 4° C prior to opening. Use aseptic technique to maintain sterility when opening product.
Notes
For research use only.
Applications Notes
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Preservative
Preservative: None. Stabilizer: None; Buffer: See application note.
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