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Competent Cell Buffer

Selected Variant

GTR19341774

Size: 100 mL

Competent Cell Buffer

Product Specifications

Product Name Alternative

Competent Cell Buffer, 60 mM Calcium Chloride, 15% (v/v) Glycerol, 10 mM Tris HCl, pH 7.5 Solution

Conjugation

Unconjugated

Concentration

1x

Purity

This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Form

Liquid (sterile filtered)

Storage Conditions

Store container at 4° C prior to opening. Use aseptic technique to maintain sterility when opening product.

Notes

For research use only.

Applications Notes

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.

Preservative

Preservative: None. Stabilizer: None; Buffer: See application note.

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