ConA-Biotin Conjugated
Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins.
Product Specifications
Applications
IHC: 1:500-1:2,500
Concentration
5 mg/ml
Storage Conditions
2-8 °C
Notes
For research use only.
Applications Notes
Histochemistry 1: 250-1:1000; WB 1:500-1:2,500; ELISA 1:500-1:2,500. Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions. For Histochemistry the tissues are processed same as for Immunohistochemistry, after blocking step biotinylated lectin is applied followed by streptavidin conjugated to enzyme and chromogen. For WB incubate membrane with blocking protein followed by biotinylated lectin, streptavidin enzyme conjugate and chromogen. For ELISA the target proteins are absorbed on ELISA plate at a concentration of 5-10 μg/ml, followed by blocking with protein solution followed by Biotinylated lectin, Streptavidin enzyme conjugate, ELISA substrate. Please refer to Histochemistry, WB and ELISA protocol for detail information.
Tested Applications
ELISA, WB
Preservative
10 mM bicarbonate, 150 mM NaCl, pH 8, 0.1 mM Calcium chloride, 0.01mM manganese chloride and 0.05% sodium azide.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
132072-ML650 100 µL
PTPN7 (Tyrosine-protein Phosphatase Non-receptor Type 7, Protein-tyrosine Phosphatase LC-PTP, Hematopoietic Protein-tyrosine Phosphatase, HEPTP) (MaxLight 650)
Sign In for Pricing
View Details