Cy5.5 acetate

Cy5.5 acetate is a CY dye, where CY stands for cyanine—an organic compound comprised of two nitrogen atoms linked with an odd number of methine units. Cyanine compounds are known for their long wavelengths, adjustable absorption and emission, high extinction coefficients, good water solubility, and relatively simple synthesis. CY dyes are frequently used for labeling proteins, antibodies, and small molecules. For protein and antibody labeling, the attachment can be achieved through a straightforward mixing reaction. Below, we outline a labeling method for proteins and antibodies, which may serve as a useful reference.