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GAPDH Monoclonal Antibody

Product Specifications

Background

GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.

Abbreviation

GAPDH

UniProt

P04406

Host

Mouse

Reactivity

Human; Mouse; Rat

Immunogen

Recombinant human GAPDH protein expressed by E.coli

Target

Aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component

Clonality

Monoclonal

Clone

7B8

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Signal Transduction; Metabolism; Cancer; Neuroscience; Isotype/Loading Controls; Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell Markers; Biochemicals; Cardiovascular; Developmental Biology

Purification

Protein A/G Purification

Concentration

1 mg/mL

Dilution

WB 1:2000-1:4000; IHC 1:200-1:400

Buffer

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Calculated Molecular Weight

36 kDa

Observed Molecular Weight

36 kDa

Isotype

IgG1

Available Sizes

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