GAPDH Monoclonal Antibody
Product Specifications
Background
GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.
Abbreviation
GAPDH
UniProt
P04406
Host
Mouse
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant human GAPDH protein expressed by E.coli
Target
Aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component
Clonality
Monoclonal
Clone
7B8
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Signal Transduction; Metabolism; Cancer; Neuroscience; Isotype/Loading Controls; Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell Markers; Biochemicals; Cardiovascular; Developmental Biology
Purification
Protein A/G Purification
Concentration
1 mg/mL
Dilution
WB 1:2000-1:4000; IHC 1:200-1:400
Buffer
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Calculated Molecular Weight
36 kDa
Observed Molecular Weight
36 kDa
Isotype
IgG1
Available Sizes
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