Epitope Tag Protein Marker Lysate
Epitope Tag Protein Marker Lysate
Product Specifications
Product Name Alternative
Flag-tag marker, MBP-tag marker, HA-tag marker, 6XHIS-tag marker, T7-tag marker, cMyc-tag marker, V5-tag marker, S-tag marker, HSV-tag marker, VSV-tag marker, Glu-Glu-tag marker, E-tag marker, Lysate marker, Protein Marker, Loading control, epitope tag marker, positive control marker
Conjugation
Unconjugated
Applications
WB: User Optimized
Concentration
5.0 mg/mL
Purity
Control lysate made in E. coli for Western blotting expresses 12 different epitope tags.
Form
Liquid
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This 12 Epitope Tag Protein Marker Lysate has been tested in western blot and is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 μL is recommended for western blotting.
Tested Applications
SDS-PAGE, WB
Preservative
Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
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