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Hydroxyphenyl Fluorescein

Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA. Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm[1].

Product Specifications

CAS Number

359010-69-8

Product Name Alternative

HPF; 3'-p- (Hydroxyphenyl) fluorescein

UNSPSC

12171500

Hazard Statement

H302, H315, H319

Target

Fluorescent Dye; Reactive Oxygen Species (ROS)

Type

Dye Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Applications

Metabolism-sugar/lipid metabolism

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/hydroxyphenyl-fluorescein.html

Purity

99.91

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

OC1=CC=C(OC2=CC3=C(C4(C5=CC=C(C=C5O3)O)C6=C(C(O4)=O)C=CC=C6)C=C2)C=C1

Molecular Formula

C26H16O6

Molecular Weight

424.40

Precautions

H302, H315, H319

References & Citations

[1]Ming-De Li, et al. Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage. J Am Chem Soc. 2018 Nov 21;140 (46) :15957-15968.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C (Powder, protect from light)

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Available Sizes

Frequently Asked Questions

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