Hydroxyphenyl Fluorescein

Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940) . Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm[1].

Product Specifications

CAS Number

[359010-69-8]

Product Name Alternative

HPF; 3'-p- (Hydroxyphenyl) fluorescein

UNSPSC

12171500

Hazard Statement

H302, H315, H319

Target

Fluorescent Dye; Reactive Oxygen Species (ROS)

Type

Dye Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Applications

Metabolism-sugar/lipid metabolism

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/hydroxyphenyl-fluorescein.html

Purity

99.91

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

OC1=CC=C(OC2=CC3=C(C4(C5=CC=C(C=C5O3)O)C6=C(C(O4)=O)C=CC=C6)C=C2)C=C1

Molecular Formula

C26H16O6

Molecular Weight

424.40

Precautions

H302, H315, H319

References & Citations

[1]Ming-De Li, et al. Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage. J Am Chem Soc. 2018 Nov 21;140 (46) :15957-15968.