Fluoroshield with PI

1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light. 3. Turn the vial upside down and remove any air bubbles. 4. Apply 2-3 drops directly of mounting medium on top of the specimen. 5. Let stand at room temperature for about 3-5 minutes in the dark. 6. Apply cover slip, carefully avoiding air bubbles. 7. The specimen is ready for visualization under microscope. 8. One can seal the edges of cover slip with nail polish or any organic medium or our Organo mounting medium. If coverslip is not sealed air bubbles will appear in few days. 9. Method for applying Coverslip: Put 1-2 drops of FLS on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. Incubate at 37°C for one hour in the dark to dry organic mounting medium. 10. For long term storage it is recommended that the slide be stored in the dark at 2-8°C.