DNA Polymerase I, Large (Klenow) Fragment

The Large, Klenow Fragment is a proteolytic product of E. coli DNA Polymerase I, which retains 5’→ 3’ polymerase activity and 3’→ 5’ exonuclease activity. It is useful for generating blunt ends by removing the 3’ overhangs and filling the 5’ overhangs. It can be also used to generate probes and second strand cDNA.