DiO Membrane Staining Kit

1. For adherent cells staining (1) Adherent cells were cultured on a sterile cover slide. (2) Remove the cover glass from the medium, absorb excess liquid but keep the surface moist. (3) Add 100 μL of Working Solution to one corner of the cover glass and gently shake to evenly cover all cells. (4) Cells were incubated at 37 °C for 2-20 mins. The reaction time can be optimized to obtain uniform labeling effect. (5) Discard Working Solution, wash the glass with PBS for 2 to 3 times. 2. For suspension cells staining (1) Adding an appropriate volume of Working Solution to re-suspension cells, the density of the cells is 1x 106 /mL. (2) The cells were incubated at 37°C for 2-20 min. The reaction time can be optimized to obtain uniform labeling effect. (3) After incubation, centrifuge at 1000-1500 rpm for 5 mins. Discard the supernatant and slowly add the growth medium again to resuspend the cells. (4) Repeat step (3) more than twice.