General Block ELISA Blocking Buffer

If fluorescence-stained cells cannot be evaluated immediately after labeling with the fluorescence probes, add Fixative to the cells at a ratio of 1:10. For example, add 100 μL Fixative to 900 μL cells and incubate for at least 15 minutes at RT in the dark. Never add Fixative until the staining and final wash steps have been completed. Fixed cells may be stored on ice or at 4°C up to 24 hours.