Glycerol mounting medium

Dehydration of specimen is not required. 1. Bring the vial to room temperature. Turn the vial upside down and open the dropper to remove any air bubbles. 2. Apply 1-3 drops of mounting medium directly on top of the specimen. 3. Place coverslip, it may be necessary to seal the edges with nail polish or any organic mounting medium like Organo mounting medium 4. Method for applying Coverslip: Put 1-2 drops of Mounting medium on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. 5. For storage it is recommended that the IHC slides can be stored at room temperature and IF slides in the dark at 2-8°C.