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Hematoxylin, PROBE

This uniquely formulated Probe hematoxylin is an excellent counterstain for in situ DNA hybridization procedure.

Product Specifications

Storage Conditions

Room Temperature

Notes

For research use only.

Applications Notes

1. After chromogen staining, wash slide 2-3X with de/di water, 2X with 2. Apply Probe hematoxylin for 2-3 minutes; wash with tap water 5-7X. 3. Soak the slides in Immuno Automation buffer, PBS or any buffer with pH 7.4-8 for 2-3 minutes. Hematoxylin changes color from purple red to blue. 4. Wash slide 2-3X with de/di water. 5. The slides can be mounted with aqueous mounting medium (ImmunoHistoMount or equivalent) for chromogens like AEC, DAB, Fast red, BCIP/INT, or Super Fast red. DAB and Super Fast can be mounted with Organic mounting mediums like Organo mount / limonene or equivalent after dehydrating the tissue with alcohols and organic solvents.

Tested Applications

IHC

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