HighYield T7 mRNA Synthesis Kit (s2UTP)

HighYield T7 mRNA Synthesis Kit (s2UTP) is designed to produce large amounts of thiouridine-modified (m) RNA via in vitro transcription with T7 RNA polymerase. The resulting internally modified (m) RNA can subsequently be used for microinjection, transfection or in vitro translation experiments. Thiouridine modifications have been shown to increase (m) RNA stability and to reduce immunogenicity. A 5'-cap structure further increases translation efficiency. 5'-capping can be achieved by a) co-transcriptional capping with cap analoga (e.g. ARCA) or b) post-transcriptional capping with capping enzymes. The kit contains sufficient reagents for 15 reactions (S-Pack) or 50 reactions (L-Pack) à 20 μl (7.5 mM GTP, 7.5 mM 2-Thio-UTP, 7.5 mM ATP, 7.5 mM CTP) . An individual optimization of 2-Thio-UTP concentration can easily be achieved with the single nucleotide format. A 20 μl reaction yields about 30-50 μg RNA after 30 min incu-bation (1 μg T7 control template, 1.4 kb RNA transcript) . Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation) ) .