HighYield T7 RNA Synthesis Kit

HighYield T7 RNA Synthesis Kit is designed to produce large amounts of RNA via in vitro transcription with T7 RNA Polymerase. It is also suitable for the incorporation of modified nucleotides (e.g. Biotin-or fluorescent-labeled NTPs or cap analoga) . The resulting RNA can subsequently be used for a variety of appli-cations such as microinjection, transfection or in vitro translation experiments, structure/function studies or probes for in situ hy-bridization and Northern Blot experiments. The kit contains sufficient reagents for 50 reactions of 20 μl each (7.5 mM each NTP) . A 20 μl reaction yields about 140-160 μg RNA after 30 min incubation (1 μg T7 control template, 1.4 kb RNA transcript) . Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation) .