His-Tag Ni-NTA Resin
His-Tag Ni-NTA Resin utilizes nickel-charged nitrilotriacetic acid on 4% agarose for high-capacity IMAC purification. Featuring four metal-binding sites, this stable resin ensures low leaching and high selectivity, even under harsh conditions involving denaturants or reducing agents.
Product Specifications
Product Name Alternative
Ni-NTA; His resin; Ni-NTA resin; His tag resin
Field of Research
Protein Biochemistry
Form
Liquid solution with 50% slurry in 20% ethanol.
Storage Conditions
Stable for one year at 2-8°C from date of shipment. Do not freeze.
Notes
For research use only.
Applications Notes
His-Tag Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+) . Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.
Preservative
Liquid in PBS buffer containing 20% ethanol.
Available Sizes
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