Hsa-miR-4477a miRNA Agomir

Components: This synthetic miRNA is based on the mature miRNA sequence. The antisense strand of the agomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. It exhibits enhanced cellular uptake, stability and regulatory activity in vivo. Directions for Use: Briefly centrifuge tubes containing miRNA agomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA agomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water) . For example: dissolve 5 nmol miRNA agomir to 20 μM using 250 μl DEPC H2O (or RNase-free water) . Pipette the solution up and down 3-5 times (or vortex briefly) . Briefly centrifuge tubes containing miRNA agomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA agomir into small volumes and store at ≤ -20°C. miRNA agomir is stable (for 6 months under the specified storage condition) . For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times