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Fatty Acid Amide Hydrolase (FAAH) Activity Fluorometric Assay Kit

Product Specifications

Type

Fluorescence method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Fatty acid amide hydrolase (FAAH) is a membrane binding protein belonging to the serine hydrolase family. FAAH hydrolyzes many important endogenous fatty acid amides, including endocannabinoid (AEA) and palmitoyl glycolamide (PEA) . FAAH plays a key role in controlling cannabinoid signaling by hydrolyzing 2arachidenol glycerol in AEA and certain tissues. Detection principle: FAAH specifically cut the substrate, the fluorophores emit fluorescence, and detect fluorescence at the excitation wavelength of 340 nm and the emission wavelength of 440 nm. Because FAAH is a serine protein, FAAHspecific inhibitors are used in the kit to inhibit enzyme activity and exclude the effects of non-specific enzyme activity.

Assay Performance Time

60 min

Sample Type

Animal tissue; cell

Detection Range

0.004-0.105 U/L

Sensitivity

0.004 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

0.2-12.2%

Intra CV

3.7-6.0%

Detection Instrument

Fluorescence Microplate Reader

Frequently Asked Questions

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