Fatty Acid Amide Hydrolase (FAAH) Activity Fluorometric Assay Kit
Product Specifications
Type
Fluorescence method
Field of Research
Glycolysis and Lipid Metabolism
Assay Principle
Fatty acid amide hydrolase (FAAH) is a membrane binding protein belonging to the serine hydrolase family. FAAH hydrolyzes many important endogenous fatty acid amides, including endocannabinoid (AEA) and palmitoyl glycolamide (PEA) . FAAH plays a key role in controlling cannabinoid signaling by hydrolyzing 2arachidenol glycerol in AEA and certain tissues. Detection principle: FAAH specifically cut the substrate, the fluorophores emit fluorescence, and detect fluorescence at the excitation wavelength of 340 nm and the emission wavelength of 440 nm. Because FAAH is a serine protein, FAAHspecific inhibitors are used in the kit to inhibit enzyme activity and exclude the effects of non-specific enzyme activity.
Assay Performance Time
60 min
Sample Type
Animal tissue; cell
Detection Range
0.004-0.105 U/L
Sensitivity
0.004 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
0.2-12.2%
Intra CV
3.7-6.0%
Detection Instrument
Fluorescence Microplate Reader
Documents
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