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Recombinant Rat IGF1

Product Specifications

Product Name Alternative

IBP1, IGF I, IGF IA, IGF IB, IGF-I, Igf1, IGF1_HUMAN, IGF1A, IGFI, IGFIA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C)

UniProt

P08025

Expression Region

49-118

Host

E.Coli

Tag

N-terminal His Tag

Applications

Western Blot, ELISA

Endotoxin

< 1 EU/µg as determined by LAL test.

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 1-3.3 ng/mL.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

9.6 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

Organism Species

Rat

Available Sizes

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