Immuno Red aqueous counter stain

In Situ hybridization, Immunohistochemistry, Immunocytochemistry. 1. After chromogen staining, wash slide 2-3X with distilled or deiononized water. 2. Apply Red aqueous counter stain for 3-5 minutes at RT. 3. Rinse slide to be mounted with Distilled or DEIONIZED water, touch the edges of slide on a paper towel to remove excess water and place the slides on a flat surface. 4. Apply 3-4 drops of Immuno In Situ mount mounting medium directly on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Wait for 5 minutes. Excess mounting medium can be removed by touching the edges of slide against a paper towel. 5. Let stand at room temperature for about 1 to 2 hours, or the slides can be heated at 37o- 40oC for 40-60 minutes. The slides are ready for visualization under a microscope, when 10X or 20X lenses are used.