Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit
Product Specifications
Target
F0F1-ATPase; ATP Synthase
Type
Colorimetric method
Field of Research
Mitochondria Function
Assay Principle
Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. The activity of mitochondrial complex V can be quantified by measure the change OD value at 340 nm.
Assay Performance Time
30 min
Sample Type
Cell
Detection Range
3.40-272.07 U/L
Sensitivity
3.40 U/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
3.0-6.5%
Intra CV
3.0-4.0%
Detection Instrument
Microplate reader
Available Sizes
Documents
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