Recombinant Feline coronavirus Non-structural protein 7a (7a), partial

Product Specifications

Product Name Alternative

(ns7a) (11 kDa protein) (Accessory protein 7a)

Abbreviation

Recombinant Feline coronavirus Non-structural protein 7a, partial

Gene Name

UniProt

P19742

Expression Region

44-75aa

Organism

Feline coronavirus (strain FIPV WSU-79/1146) (FCoV)

Target Sequence

VPDSSLRVNCLQLLKPDCLDFNILHKVLAETR

Tag

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

May function in the formation of membrane-bound replication complexes or in the assembly of the virus.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

19.7 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.