Lysosome Green Probes

1. Prepare the working solution Add Lysosome Red Probes to the cell culture medium at the ratio of 1:10,000 - 1:20,000. The final concentration is 50-100 nM. The working solution can be warmed at 37°C before use. 2. Label the lysosomes For adherent cells, discard the cell medium and wash with 1 x PBS. Adding the working solution, and incubate the cells at 37°C for 30 mins to 2 hours. Discard the working solution, wash with 1 x PBS for three times, and then photographed under a fluorescence microscope. For suspension cells, centrifuge to obtain a cell pellet and aspirate the supernatant. Resuspend the cells gently in probe-containing medium, and incubate the cells at 37°C for 30 mins to 2 hours. Centrifuge to obtain a cell pellet and aspirate the supernatant, wash with 1 x PBS for three times, and then photographed under a fluorescence microscope. Notice 1. If the staining effect is not good, the concentration of the probe in the working fluid can increase the concentration of the working solution , or can extend the reaction time. 2. In order to reduce the background, pleasee use lower concentration probes. 3. Take photos quickly, because the dye is easy to quench.